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Home >> Antibodies >> Ep-CAM / CD326 (Epithelial Marker)

Ep-CAM / CD326 (Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone EGP40/1556R]

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Catalog No Format Size Price (USD)
4072-RBM16-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
4072-RBM16-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
4072-RBM16-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
4072-RBM16-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
4072-RBM16-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 4072
Human SwissProt P16422
Human Unigene 542050
Human Gene Symbol TACSTD1
Human Chromosome Location 2p21
Synonyms Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2
Immunogen Recombinant human TACSTD1 protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 40-43kDa
Cellular Localization Cell surface
Species Reactivity Human. Does not react with mouse and rat. Others not known.
    

Specificity & Comments

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HT29 cells or breast tumor

Supplied As

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Bjork, P., Jonsson, U., Svedberg, H., Larsson, K., Lind, P., Dillner, J., Hedlund, G., Dohlsten, M. and Kalland, T. 1993. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glyco- protein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241.
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