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Home >> Antibodies >> Chromogranin A / CHGA (Neuroendocrine Marker)

Chromogranin A / CHGA (Neuroendocrine Marker)

Recombinant Rabbit Monoclonal Antibody [Clone CHGA/1731R]

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Catalog No Format Size Price (USD)
1113-RBM11-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
1113-RBM11-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
1113-RBM11-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
1113-RBM11-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
1113-RBM11-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 1113
Human SwissProt P10645
Human Unigene 150793
Human Gene Symbol CHGA
Human Chromosome Location 14q32.12
Synonyms Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; Vasostatin I or II
Immunogen Recombinant human full-length Chromogranin A protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 68-75kDa
Cellular Localization Finely granular cytoplasmic
Species Reactivity Human. Does not react with rat. Others not known.
         

Specificity & Comments

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

PC12 cells. Adrenal gland, bowel, parathyroid, pancreas, or pheochromocytoma.

Supplied As

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Konecki DS et. al. J Biol Chem 1987;262:17026-30.
  2. Lloyd RV et. al. Am J Pathol 1988; 130:296-304.
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