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Home >> Antibodies >> CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)

CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)

Mouse Monoclonal Antibody [Clone CFTR/1643]

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Catalog No Format Size Price (USD)
1080-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
1080-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
1080-MSM3-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
1080-MSM3-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
1080-MSM3-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 1080
Human SwissProt P13569
Human Unigene 489786, 621460, 661104
Human Gene Symbol CFTR
Human Chromosome Location 7q31.2
Synonyms ABC35; ATP Binding Cassette Superfamily C Member 7 (ABCC7); cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; Cystic Fibrosis Transmembrane Conductance Regulator; MRP7; TNR CFTR
Immunogen Recombinant human CFTR fragment (aa258-385) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b
Mol. Weight of Antigen 165-170kDa
Cellular Localization Cell Surface and Cytoplasmic
Species Reactivity Human. Others not known.
    

Specificity & Comments

Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Known Applications & Suggested Dilutions

Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

MOLT-4 cells. Pancreas, Kidney or Placenta.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. s Riordan, J.R., et al. 1989. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073.
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