2 Union Square, Union City, CA 94587 contact@NeoBiotechnologies.com 510-376-5603

Home >> Antibodies >> CD31 / PECAM-1 (Endothelial Cell Marker)

CD31 / PECAM-1 (Endothelial Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone C31/1395R]

PDF Version  Printable Version
Catalog No Format Size Price (USD)
5175-RBM11-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
5175-RBM11-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
5175-RBM11-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
5175-RBM11-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
5175-RBM11-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 5175
Human SwissProt P16284
Human Unigene 376675 & 514412
Human Gene Symbol PECAM1
Human Chromosome Location 17q23.3
Synonyms EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
Immunogen Human recombinant full-length CD31 protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen ~100kDa (endothelium) and ~130kDa (platelets)
Cellular Localization Cell surface and cytoplasmic
Species Reactivity Human. Others not known.
    

Specificity & Comments

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Jurkat cells. Tonsil or Angiosarcoma.

Supplied As

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).
DOC#01395