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Home >> Antibodies >> CD27 (Tumor Necrosis Factor Receptor Superfamily 7)

CD27 (Tumor Necrosis Factor Receptor Superfamily 7)

Mouse Monoclonal Antibody [Clone LPFS2/1611]

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Catalog No Format Size Price (USD)
939-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
939-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
939-MSM2-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
939-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
939-MSM2-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 939
Human SwissProt P26842
Human Unigene 355307
Human Gene Symbol CD27
Human Chromosome Location 12p13
Synonyms LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7
Immunogen Recombinant human CD22 protein (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1
Mol. Weight of Antigen 120kDa
Cellular Localization Cell Surface
Species Reactivity Human. Others not-tested.
    

Specificity & Comments

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Human peripheral blood lymphocytes. Human tonsil and lymph node.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. s De Rie MA, Cairo I, Van Lier RA, Bos JD. Expression of the T-cell activation antigens CD27 and CD28 in normal and psoriatic skin. Clin Exp Dermatol. 1996 Mar;21(2):104-11.
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