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Home >> Antibodies >> Blood Group Antigen B (CD173)

Blood Group Antigen B (CD173)

Mouse Monoclonal Antibody [Clone HEB-20]

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Catalog No Formulation Size Price (USD)  
28-MSM7-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
28-MSM7-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
28-MSM7-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
28-MSM7-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
28-MSM7-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 28
Human SwissProt P16442
Human Unigene 654423
Human Gene Symbol ABO
Human Chromosome Location 9q34.2
Synonyms A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT
Immunogen Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen Multiple
Cellular Localization Cell Surface
Species Reactivity Human. Others-not known.

Specificity & Comments

The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Known Applications & Suggested Dilutions

Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

KG1 cells or human colorectal carcinoma tissues.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.


This antibody is available for research use only and is not approved for use in diagnosis.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Vanak J, Drimalova D, Smyslova O, Nĕmec M, Viklický V, Wisniewski K. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88.