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Home >> Antibodies >> Androgen Receptor (Marker of Androgen Dependence)

Androgen Receptor (Marker of Androgen Dependence)

Mouse Monoclonal Antibody [Clone AR441]

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Catalog No Format Size Price (USD)
367-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
367-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
367-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
367-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
367-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 367
Human SwissProt P10275
Human Unigene 496240
Human Gene Symbol AR
Human Chromosome Location Xq12
Synonyms AIS; Dihydrotestosterone receptor (DHTR); HUMARA; HYSP1; Kennedy disease (KD); Nuclear receptor subfamily 3 group C member 4 (NR3C4); SMAX1; Spinal and bulbar muscular atrophy (SBMA); Testicular Feminization (TFM)
Immunogen A synthetic peptide, aa 302-318 (STEDTAEYSPFKGGYTK) of human AR
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 110kDa
Cellular Localization Nuclear
Species Reactivity Human. Does not react with mouse. Others-not known
    

Specificity & Comments

Recognizes a protein of 110kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This MAb is superb for staining of formalin/paraffin tissues.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

LNCap cells or Prostate carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sJanne OA, et al. Androgen receptor and mechanism of androgen action. Ann Med 1993; 25:83
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