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Home >> Antibodies >> Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker)

Mouse Monoclonal Antibody [Clone AAT/1378]

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Catalog No Format Size Price (USD)
5265-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
5265-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
5265-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
5265-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
5265-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 5265
Human SwissProt P01009
Human Unigene 525557
Human Gene Symbol SERPINA1
Human Chromosome Location 14q32.1
Synonyms A1AT; AAT; Alpha 1 antiproteinase Alpha 1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; alpha1 proteinase inhibitor; Alpha1AT; Dom1; Serine (or cysteine) proteinase inhibitor clade A member 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1a; Serpin peptidase inhibitor clade A member 1; Serpina1; Short peptide from AAT; SPAAT; Spi1-1
Immunogen Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1
Mol. Weight of Antigen 54kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others-not known.
         

Specificity & Comments

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
western Blotting (0.5-1.0ug/ml)
Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 Cells. Tonsil, Hepatocellular Carcinoma or Histiocytoma.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Atkinson, G.W., Ann Clin Lab Sci 3:345-352, 1973.Kyaw-Myint, T.O., et al., Clin Chem Acta 59:51-54, 1975.
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