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Home >> Antibodies >> Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker)

Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker)

Mouse Monoclonal Antibody [Clone AACT/1452]

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Catalog No Format Size Price (USD)
12-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
12-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
12-MSM2-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
12-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
12-MSM2-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 12
Human SwissProt P01011
Human Unigene 534293, 710488
Human Gene Symbol SERPINA3
Human Chromosome Location 14q32.1
Synonyms SERPINA3; AACT; ACT; Alpha-1-antichymotrypsin; Antichymotrypsin; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; Serine (or cysteine) proteinase inhibitor clade A member 3; Serine proteinase inhibitor clade A member 3; Serpin A3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3
Immunogen Recombinant human Antichymotrypsin (AACT) protein fragment (aa49-187) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1
Mol. Weight of Antigen 65-76kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others-not known.
    

Specificity & Comments

It recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of α 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa Cells. Tonsil, Pancreas or Histiocytoma.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sMiyake, H., et al. 2001. Value of prostate specific antigen α1-antichymotrypsin complex for the detection of prostate cancer in patients with a PSA level of 4.1-10.0 ng/mL: comparison with PSA-related parameters. Int. J. Urol. 8: 589-593.
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