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Home >> Antibodies >> Alkaline Phosphatase (Tissue-Nonspecific)

Alkaline Phosphatase (Tissue-Nonspecific)

Mouse Monoclonal Antibody [Clone ALPL/597]

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Catalog No Format Size Price (USD)
249-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
249-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
249-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
249-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
249-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 249
Human SwissProt P05186
Human Unigene 75431
Human Gene Symbol ALPL
Human Chromosome Location 1p36.12
Synonyms Alkaline phosphatase, germ cell; Alkaline phosphatase, testicular and thymus; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase, tissue-nonspecific; ALPG; ALPL; Germ cell alkaline phosphatase; Testicular and thymus alkaline phosphatase; Tissue non-specific alkaline phosphatase; Tissue nonspecific ALP (TNAP or TNSALP)
Immunogen Recombinant human ALPL protein
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 55kDa
Cellular Localization Cell Surface
Species Reactivity Human and Cow. Others not known.
                   

Specificity & Comments

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1:50-1:100 for 30 min at Room Temp)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Intestine

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Griffin, C.A., et al. 1987. Human placental and intestinal alkaline phosphatase genes map to 2q34-q37. Am. J. Hum. Genet. 41: 1025-1034.
  2. sMoss, D.W. 1987. Diagnostic aspects of alkaline phosphatase and its isoenzymes. Clin. Biochem. 20: 225-230.
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