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Home >> Antibodies >> Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone GM022]

Catalog No Formulation Size Price (USD)  
250-MSM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
250-MSM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
250-MSM4-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
250-MSM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
250-MSM4-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
250-MSM4-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 250
Human SwissProt P05187
Human Unigene 284255
Human Gene Symbol ALPP
Human Chromosome Location 2q37.1
Synonyms Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1
Immunogen Recombinant human PLAP protein
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 70kDa
Cellular Localization Cytoplasmic and cell surface
Species Reactivity Human, Others not known.

Specificity & Comments

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.Ā

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 cells. Placenta or seminoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Wick, MR, et al. 1987; Hum Pathol. 18(9):946-54.
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