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Home >> Antibodies >> Adipophilin / Perilipin-2 (Marker of Obesity)

Adipophilin / Perilipin-2 (Marker of Obesity)

Mouse Monoclonal Antibody [Clone ADFP/1494]

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Catalog No Format Size Price (USD)
123-MSM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
123-MSM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
123-MSM4-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
123-MSM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
123-MSM4-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 123
Human SwissProt Q99541
Human Unigene 3416
Human Gene Symbol PLIN2
Human Chromosome Location 9p22.1
Synonyms Adipophilin; ADFP; Adipose differentiation-related protein (ADRP); Perilipin-2 (PLIN2)
Immunogen Recombinant human Adipophilin protein fragment (aa249-376) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 48kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others not known.
    

Specificity & Comments

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Known Applications & Suggested Dilutions

ELISA (For coating, order Ab without BSA)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Kern, P.A., et al. 2004. Perilipin expression in human adipose tissue is elevated with obesity. J. Clin. Endocrinol. Metab. 89: 1352-1358.
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