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Home >> Antibodies >> Adiponectin (Marker of Obesity)

Adiponectin (Marker of Obesity)

Mouse Monoclonal Antibody [Clone ADPN/1370]

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Catalog No Format Size Price (USD)
9370-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
9370-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
9370-MSM3-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
9370-MSM3-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
9370-MSM3-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 9370 (Human)
Human SwissProt Q15848 (Human)
Human Unigene 80485 (Human)
Human Gene Symbol ADIPOQ
Human Chromosome Location 3q27
Synonyms Adiponectin; Adipocyte complement-related 30kDa protein (ACRP30); Adipocyte-specific secretory protein; Adiponectin, C1Q and collagen domain containing (ACDC); ADIPOQ; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM-1; Gelatin-binding protein 28 (GBP28)
Immunogen Recombinant human Adiponectin protein fragment
Host / Ig Isotype Mouse / IgG2b
Mol. Weight of Antigen 26-30kDa
Cellular Localization Cytoplasmic & Cell Surface
Species Reactivity Human. Others not known.
    

Specificity & Comments

This MAb reacts with adiponectin, an adipocytokine. Adipocytokines are hormones produced in adipose tissue. Adiponectin is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma adiponectin levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women adiponectin levels tend to be higher than in men, which may be due to androgens suppressing adiponectin levels. Furthermore adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low adiponectin levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

Known Applications & Suggested Dilutions

ELISA (For coating, use antibody without BSA),Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa cells or Kidney

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sDos Santos, E., et al. 2008. Adiponectin mediates an antiproliferative response in human MDA-MB 231 breast cancer cells. Oncol. Rep. 20: 971-977.
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