|Catalog No||Formulation||Size||Price (USD)|
|MSM1X-445-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||129.00|
|MSM1X-445-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||329.00|
|MSM1X-445-P2||Purified Ab with BSA and Azide at 200ug/ml||200 ug||459.00|
|MSM1X-445-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||359.00|
|MSM1X-445-P2ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||200 ug||489.00|
|MSM1X-445-IHC7||Prediluted Ab for IHC||7.0 ml||329.00|
|Human Entrez Gene ID||58, 59, 70|
|Human SwissProt||P62736, P68032, P68133|
|Human Unigene||118127, 1288, 500483|
|Human Gene Symbol||ACTA1 (Skeletal); ACTA2 (Smooth); ACTG2 (Smooth)|
|Human Chromosome Location||1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)|
|Synonyms||ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1|
|Immunogen||SDS extract of human myocardium.|
|Host / Ig Isotype||Mouse / IgG1, kappa|
|Mol. Weight of Antigen||Multiple|
|Species Reactivity||Human, Rabbit and Rat. Others-not known.|
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.ĀIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Muscle or sarcoma
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.