2 Union Square, Union City, CA 94587 contact@NeoBiotechnologies.com 510-376-5603

Home >> Antibodies >> ASRGL1 (Asparaginase Like 1)

ASRGL1 (Asparaginase Like 1)

Mouse Monoclonal Antibody [Clone CRASH/1289]

PDF Version  Printable Version
Catalog No Format Size Price (USD)
80150-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
80150-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
80150-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
80150-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
80150-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 80150 (Human)
Human SwissProt Q7L266 (Human)
Human Unigene 535326 (Human)
Human Gene Symbol ASRGL1
Human Chromosome Location 11q12.3
Synonyms ALP1; ASGL1; Asparaginase like 1; ASRGL1; beta-aspartyl-peptidase; CRASH; isoaspartyl dipeptidase; isoaspartyl peptidase/L-asparaginase; L asparaginase; L asparagine amidohydrolase
Immunogen Recombinant human full-length ASRGL1
Host / Ig Isotype Mouse / IgG1
Mol. Weight of Antigen 17-25kDa
Cellular Localization Cytoplasmic and Nuclear
Species Reactivity Human. Others not known.
              

Specificity & Comments

ASRGL1 (Asparaginase-like protein 1), also known as CRASH, is a 308 amino acid protein belonging to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. ASRGL1 is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa cells. Fallopian Tube, Endometrium, Uterus or Cervix.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Evtimova, V., et al. 2004. Identification of CRASH, a gene deregulated in gynecological tumors. Int. J. Oncol. 24: 33-41.
DOC#01289