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Home >> Antibodies >> AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker)

AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker)

Mouse Monoclonal Antibody [Clone AMPD3/901]

Catalog No Formulation Size Price (USD)  
272-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
272-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
272-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
272-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
272-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
272-MSM1-IHC7 Prediluted Ab for IHC 7.0 ml 329.00
Human Entrez Gene ID 272
Human SwissProt Q01432
Human Unigene 501890
Human Gene Symbol AMPD3
Human Chromosome Location 11p15.4
Synonyms Adenosine monophosphate deaminase (isoform E); AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; Ampd3; Erythrocyte AMP deaminase; Erythrocyte specific AMP deaminase; Erythrocyte specific protein; Erythrocyte type AMP deaminase; Myoadenylate deaminase
Immunogen Recombinant full-length human AMDP3 protein
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen ~90kDa
Cellular Localization Cell Surface
Species Reactivity Human, Others-not known.

Specificity & Comments

It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunocytochemistry (1-2.0ug/ml for 30 minutes at RT)
mmunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

RBC. Fetal liver; Spleen or Placenta

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Sabina RL, Waldenström A, Ronquist G. The contribution of Ca+ calmodulin activation of human erythrocyte AMP deaminase (isoform E) to the erythrocyte metabolic dysregulation of familial phosphofructokinase deficiency. Haematologica. 2006;91(5):652-5.
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