|Catalog No||Formulation||Size||Price (USD)|
|272-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||129.00|
|272-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||329.00|
|272-MSM1-P2||Purified Ab with BSA and Azide at 200ug/ml||200 ug||459.00|
|272-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||359.00|
|272-MSM1-P2ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||200 ug||489.00|
|272-MSM1-IHC7||Prediluted Ab for IHC||7.0 ml||329.00|
|Human Entrez Gene ID||272|
|Human Gene Symbol||AMPD3|
|Human Chromosome Location||11p15.4|
|Synonyms||Adenosine monophosphate deaminase (isoform E); AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; Ampd3; Erythrocyte AMP deaminase; Erythrocyte specific AMP deaminase; Erythrocyte specific protein; Erythrocyte type AMP deaminase; Myoadenylate deaminase|
|Immunogen||Recombinant full-length human AMDP3 protein|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||~90kDa|
|Cellular Localization||Cell Surface|
|Species Reactivity||Human, Others-not known.|
It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 220.127.116.11. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunocytochemistry (1-2.0ug/ml for 30 minutes at RT)
mmunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
RBC. Fetal liver; Spleen or Placenta
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.