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Home >> Antibodies >> ALK (Anaplastic Lymphoma Kinase) / CD246

ALK (Anaplastic Lymphoma Kinase) / CD246

Mouse Monoclonal Antibody [Clone ALK/1503]

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Catalog No Format Size Price (USD)
238-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
238-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
238-MSM3-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
238-MSM3-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
238-MSM3-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 238
Human SwissProt Q9UM73
Human Unigene 654469
Human Gene Symbol ALK
Human Chromosome Location 2p23
Synonyms ALK Tyrosine Kinase Receptor, ALK/NPM1 fusion gene, Anaplastic lymphoma kinase Ki1, Anaplastic Lymphoma Kinase p80, anaplastic lymphoma receptor tyrosine kinase, CD246, mutant anaplastic lymphoma kinase, NBLST3, Tcrz, TFG/ALK
Immunogen Recombinant human ALK protein fragment (aa1360-1460) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 80kDa (hybrid); 200kDa (wild type)
Cellular Localization Cytoplasmic and nuclear
Species Reactivity Human. Others-not known.
    

Specificity & Comments

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.

Positive Control

Anaplastic Large Cell Lymphoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sPulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997;89:1394-404.
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