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Home >> Antibodies >> ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker)

ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker)

Recombinant Mouse Monoclonal Antibody [Clone r57]

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Catalog No Format Size Price (USD)
5443-MSM6-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
5443-MSM6-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
5443-MSM6-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
5443-MSM6-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
5443-MSM6-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 5443
Human SwissProt P01189
Human Unigene 1897
Human Gene Symbol POMC
Human Chromosome Location 2p23.3
Synonyms Adrenocorticotropin; alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin; beta-Endorphin; beta or gamma Lipotropin (LPH); CLIP; Met Enkephalin; POC; POMC
Immunogen N-terminal fragment of human ACTH conjugated to KLH
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
Cellular Localization Cytoplasm and Secreted
Species Reactivity Human, Mouse and Rat. Expected to show a broad species reactivity.
    

Specificity & Comments

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Known Applications & Suggested Dilutions

ELISA (For coating, order Ab without BSA)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Normal pituitary gland or pituitary tumor.

Supplied As

200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. s1. Hsu DW et. al. American Journal of Pathology, 1991, 138(4):897-909.
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